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Simultaneous Multiplane Imaging in Digital Holographic Microscopy

  Articoli su Riviste JCR/ISI  (anno 2011)

Autori:  Paturzo M., Finizio A., Ferraro P

Affiliazione Autori:  CNR - Istituto Nazionale di Ottica, 80078 Pozzuoli, NA, Italy

Riassunto:  Reduced depth of field is a severe limitation in microscopy since, at high magnification, only a portion of the imaged volume along the optical axis is in good focus at once. We demonstrate that, adopting a numerical quadratic deformed diffraction grating into the reconstruction process of digital holograms, it is possible to image three planes at different depths simultaneously, gaining flexibility dispensing with a physical grating. Moreover, multiplexed holograms recorded with two different wavelengths can be obtained in focus simultaneously by a single reconstruction. Description of the method and applications for macroscopic and microscopic biological samples are reported.

Volume n.:  7 (1)      Pagine da: 24  a: 28
Ulteriori informazioni:  Manuscript received July 12, 2010; revised October 05, 2010; accepted October 05, 2010. Date of current version January 12, 2011. The research leading to these results has beedn supported by the European Community\'s Seventh Framework Programme FP7/2007-2013 under Grant Agreement 216105 (\"Real 3D\" Project)
DOI: 10.1109/JDT.2010.2087007

*Impact Factor della Rivista: (2011) 2.280   *Citazioni: 13
data tratti da "WEB OF SCIENCE" (marchio registrato di Thomson Reuters) ed aggiornati a:  19/05/2019

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