INO
CNR
vai_a_storia   vai_a_organizzazione   vai_a_sedi   vai_a_personale   Area Riservata
    English English Version  
 
 

Thermal Transitions of Fibrillar Collagen Unveiled by Second-Harmonic Generation Microscopy of Corneal Stroma

  Articoli su Riviste JCR/ISI  (anno 2012)

Autori:  Matteini P., Cicchi R., Ratto F., Kapsokalyvas D., Rossi F., de Angelis M., Pavone FS., Pini R

Affiliazione Autori:  Institute of Applied Physics ‘‘Nello Carrara’’, National Research Council, Sesto Fiorentino, Italy; National Institute of Optics, National Research Council, Florence, Italy; LENS, European Laboratory for Non-Linear Spectroscopy, Sesto Fiorentino, Italy

Riassunto:  The thermal transitions of fibrillar collagen are investigated with second-harmonic generation polarization anisotropy microscopy. Second-harmonic generation images and polarization anisotropy profiles of corneal stroma heated in the 35-80 degrees C range are analyzed by means of a theoretical model that is suitable to probe principal intramolecular and interfibrillar parameters of immediate physiological interest. Our results depict the tissue modification with temperature as the interplay of three destructuration stages at different hierarchical levels of collagen assembly including its tertiary structure and interfibrillar alignment, thus supporting and extending previous findings. This method holds the promise of a quantitative inspection of fundamental biophysical and biochemical processes and may find future applications in real-time and postsurgical functional imaging of collagen-rich tissues subjected to thermal treatments.

Rivista/Giornale:  BIOPHYSICAL JOURNAL
Volume n.:  103 (6)      Pagine da: 1179  a: 1187
Ulteriori informazioni:  This work has been partially supported by the FP7 NoE Photonics 4 Life and by the NANO-CHROM Project of the Health Board of Tuscany.
DOI: 10.1016/j.bpj.2012.07.055

*Impact Factor della Rivista: (2012) 3.668   *Citazioni: 28
data tratti da "WEB OF SCIENCE" (marchio registrato di Thomson Reuters) ed aggiornati a:  21/07/2019

Riferimenti visionabili in IsiWeb of Knowledge: (solo per sottoscrittori)
Per visualizzare la scheda dell'articolo su IsiWeb: Clicca qui
Per visualizzare la scheda delle Citazioni dell'articolo su IsiWeb: Clicca qui

INO © Istituto Nazionale di Ottica - Largo Fermi 6, 50125 Firenze | Tel. 05523081 Fax 0552337755 - P.IVA 02118311006     P.E.C.    Info