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3D visualization and biovolume estimation of motile cells by digital holography

  Articoli su Riviste JCR/ISI  (anno 2014)

Autori:  Merola F., Miccio L., Memmolo P., Di Caprio G., Coppola G., Netti P

Affiliazione Autori:  CNR-INO CNR-IMM IIT-CRIB

Riassunto:  For the monitoring of biological samples, physical parameters such as size, shape and refractive index are of crucial importance. However, up to now the morphological in-vitro analysis of in-vitro cells has been limited to 2D analysis by classical optical microscopy such as phase-contrast or DIC. Here we show an approach that exploits the capability of optical tweezers to trap and put in self-rotation bovine spermatozoa flowing into a microfluidic channel. At same time, digital holographic microscopy allows to image the cell in phase-contrast modality for each different angular position, during the rotation. From the collected information about the cell

Rivista/Giornale:  PROCEEDINGS OF SPIE
Volume n.:  9129      Pagine da: 91291W  a: 91291W
DOI: 10.1117/12.2052836

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