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Probing lysine mono-methylation in histone H3 tail peptides with an abiotic receptor coupled to a non-plasmonic resonator

  Articoli su Riviste JCR/ISI  (anno 2017)

Autori:  Bontempi N., Biavardi E., Bordiga D., Candiani G., Alessandri I., Bergese P., Dalcanale E

Affiliazione Autori:  Department of Mechanical and Industrial Engineering, Chemistry for Technologies Laboratory, University of Brescia, INSTM UdR Brescia, Via Branze 38, Brescia, 25123, Italy; Department of Chemistry, Life Science and Environmental Sustainability, University of Parma, INSTM UdR Parma, Parco Area delle Scienze 17/A, Parma, 43124, Italy; Department of Molecular and Translational Medicine, University of Brescia, INSTM UdR Brescia, Viale Europa 11, Brescia, 25123, Italy; Department of Chemistry, Materials and Chemical Engineering G. Natta, Polytechnic of Milan, INSTM UdR Milano, Via Mancinelli 7, Milano, 20131, Italy

Riassunto:  Binder and effector molecules that allow studying and manipulating epigenetic processes are of biological relevance and pose severe technical challenges. We report the first example of a synthetic receptor able to recognize mono-methylated lysines in a histone H3 tail peptide, which has relevant functions in epigenetic regulation. Recognition is robust and specific regardless of the position and the number of mono-methylated lysines along the polypeptide chain. The peptide is first captured in solution by a tetraphosphonate cavitand (Tiiii) that selectively binds its Lys-NMe+ moieties. Separation from solution and detection of the peptide-Tiiii complexes is then enabled in one single step by an all dielectric SiO2-TiO2 core-shell resonator (T-rex), which captures the complex and operates fully reproducible signal transduction by non-plasmonic surface enhanced Raman scattering (SERS) without degrading the complex. The realized abiotic probe is able to distinguish multiple mono-methylated peptides from the single mono-methylated ones.

Rivista/Giornale:  NANOSCALE
Volume n.:  9 (25)      Pagine da: 8639  a: 8646
Ulteriori informazioni:  This work was supported by SUPRANANO (INSTM-Regione Lombardia project). Centro Intefacolta di Misure \"G. Casnati\" of the University of Parma is acknowledged for the use of NMR facilities. We thank Matteo Ferroni for support in SEM analysis.
DOI: 10.1039/c7nr02491f

*Impact Factor della Rivista: (2017) 7.233   *Citazioni: 11
data tratti da "WEB OF SCIENCE" (marchio registrato di Thomson Reuters) ed aggiornati a:  19/05/2019

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